LOCUS       40924_13945        5901 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral vector pCX4bleo DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Akagi T, Sasai K, Hanafusa H.
  TITLE     Refractory nature of normal human diploid fibroblasts with respect 
            to oncogene-mediated transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 100 (23), 13567-13572 (2003)
  PUBMED    14597713
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute, 
            Molecular Oncology; Furuedai 6-2-4, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax:81-6-6871-7521)
REFERENCE   3  (bases 1 to 5901)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5901)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2003"; volume: "100"; issue: "23"; pages:
            "13567-13572"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute, Molecular 
            Oncology"; volume: " Furuedai 6-2-4, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax"; pages: 
            "81-6-6871-7521"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label=R-U5 region of MuLV 5' LTR
                     /note="R-U5 region of MuLV 5' LTR"
     misc_feature    826..1183
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1242..1658
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1668..2042
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2044..2106
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    2154..2715
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2718..3089
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3196..3787
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4088..4676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4843..5700)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5701..5805)
                     /label=AmpR promoter
ORIGIN
        1 tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg
       61 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
      121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac
      301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgcta gagaaccatc
      481 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
      601 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
      661 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
      721 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
      781 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
      961 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     1021 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat
     1081 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     1141 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     1201 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
     1261 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1321 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1381 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1441 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1501 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1561 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1621 cctcccttta tccagccctc actccttctc taggcgcccc catatggcca tatgagatct
     1681 tatatggggc acccccgccc cttgtaaact tccctgaccc tgacttgaca agagttacta
     1741 acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga
     1801 gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc
     1861 gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa cctcgctgga
     1921 aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac ggcatcgcag
     1981 cttggataca cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac
     2041 tgccggatcc cagtgtggtg gtacgggaat tccggaccga cgcgtgcggc cgcggtaaca
     2101 attgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc
     2161 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2221 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2281 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2341 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     2401 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     2461 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     2521 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     2581 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     2641 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     2701 aaaaacacga taataccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     2761 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     2821 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     2881 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     2941 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     3001 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     3061 gcgtgcactt cgtggccgag gagcaggact gaccgacgcc gaccaacacc gccggtccga
     3121 cgcggcccga cgggtccgag gcatcgtcga cacgataaaa taaaagattt tatttagtct
     3181 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     3241 gccattttgg aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     3301 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     3361 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     3421 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     3481 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     3541 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     3601 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     3661 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     3721 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     3781 tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt attaacatta
     3841 aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3901 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4501 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     4561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgcgg ccggccgcaa
     4801 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4861 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4921 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4981 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5041 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5101 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5161 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5221 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5281 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5341 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5401 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     5461 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5521 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5581 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5641 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5701 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5761 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5821 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5881 tatcacgagg ccctttcgtc c
//
